The M-Rogue Research Learning Center publishes practical, method-focused guides for laboratory researchers working with research peptides. The articles cover reading certificates of analysis, HPLC purity testing, LC-MS identity confirmation, lyophilized peptide handling and the chemistry of the compounds in our catalog. All products are for laboratory and in vitro research use only (RUO).

Analytical Testing Guides

  • How to Read and Verify a Peptide COA: what a certificate of analysis records for a specific sample, including the lot number, purity and net content, the chromatogram, any mass spectrum, and how to verify a certificate with the issuing laboratory.
  • HPLC in Peptide Analysis: how reversed-phase HPLC separates a peptide from synthesis-related impurities, why detection at 214 nm is used, how purity is calculated from peak areas, and how the components of a blend resolve as separate peaks.
  • LC-MS vs HPLC: How Molecular Identity is Confirmed: the difference between purity and identity, and between retention-time identity and mass-spectrometry identity, with a note on which method each of our posted COAs uses.

Handling and Compound Guides

Using These Guides

The guides are written to help you interpret documentation and handle materials consistently in the lab. Each product page links to the guides that apply to it, and every posted certificate is listed on our Certificates of Analysis page. If something isn’t covered here, contact us.

The Learning Center is for educational and laboratory reference only. It does not describe or endorse any use of these materials outside the laboratory.